The complete genome sequence of Xanthomonas theicola, the causal agent of canker on tea plants, reveals novel secretion systems in clade-1 xanthomonads.
Identifieur interne : 000030 ( Main/Exploration ); précédent : 000029; suivant : 000031The complete genome sequence of Xanthomonas theicola, the causal agent of canker on tea plants, reveals novel secretion systems in clade-1 xanthomonads.
Auteurs : Ralf Koebnik [France] ; Daiva Burokiene [Lituanie] ; Claude Bragard [Belgique] ; Christine Chang [Oman] ; Marion Fischer-Le Saux [France] ; Roland Kölliker [Suisse] ; Jillian Lang [États-Unis] ; Jan Leach [États-Unis] ; Emily Luna [États-Unis] ; Perrine Portier [France] ; Angeliki Sagia [France, Oman] ; Janet Ziegle [Oman] ; Stephen Philip Cohen [États-Unis] ; Jonathan Jacobs [États-Unis]Source :
- Phytopathology [ 0031-949X ] ; 2020.
Abstract
Xanthomonas theicola is the causal agent of bacterial canker on tea plants. There is no complete genome sequence available for X. theicola, a close relative of the species X. translucens and X. hyacinthi, thus limiting basic research for this group of pathogens. Here we release a high-quality complete genome sequence for the X. theicola type strain, CFBP 4691T. Single-molecule real-time sequencing with a mean coverage of 264X revealed two contigs of 4,744,641 bp (chromosome) and 40,955 bp (plasmid) in size. Genome mining revealed the presence of non-ribosomal peptide synthases, two CRISPR systems, the Xps type 2 secretion system, and the Hrp type 3 secretion system. Surprisingly, this strain encodes an additional type 2 secretion system and a novel type 3 secretion system with enigmatic function, hitherto undescribed for xanthomonads. Four type 3 effector genes were found on complete or partial transposons, suggesting a role of transposons in effector gene evolution and spread. This genome sequence fills an important gap to better understand the biology and evolution of the early-branching xanthomonads, also known as clade-1 xanthomonads.
DOI: 10.1094/PHYTO-07-20-0273-SC
PubMed: 32997607
Affiliations:
- Belgique, France, Lituanie, Oman, Suisse, États-Unis
- Colorado, Pays de la Loire, Province du Brabant wallon, Région wallonne
- Angers, Louvain-la-Neuve
- Université catholique de Louvain, Université d'Angers
Links toward previous steps (curation, corpus...)
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Jan.Leach@colostate.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Luna, Emily" sort="Luna, Emily" uniqKey="Luna E" first="Emily" last="Luna">Emily Luna</name><affiliation wicri:level="2"><nlm:affiliation>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado, United States; emily.peachey@colostate.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Portier, Perrine" sort="Portier, Perrine" uniqKey="Portier P" first="Perrine" last="Portier">Perrine Portier</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Recherche en Horticulture et Semences, 98754, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, Angers, Pays de la Loire, France; 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sagia.angeliki@gmail.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>University of Crete, 37777, Department of Biology, Heraklion, Crete</wicri:regionArea><wicri:noRegion>Crete</wicri:noRegion></affiliation></author><author><name sortKey="Ziegle, Janet" sort="Ziegle, Janet" uniqKey="Ziegle J" first="Janet" last="Ziegle">Janet Ziegle</name><affiliation wicri:level="1"><nlm:affiliation>Pacific Biosciences Inc, 105480, Menlo Park, California, United States; jziegle@pacificbiosciences.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Pacific Biosciences Inc, 105480, Menlo Park, California</wicri:regionArea><wicri:noRegion>California</wicri:noRegion></affiliation></author><author><name sortKey="Cohen, Stephen Philip" sort="Cohen, Stephen Philip" uniqKey="Cohen S" first="Stephen Philip" last="Cohen">Stephen Philip Cohen</name><affiliation wicri:level="1"><nlm:affiliation>The Ohio State University, 2647, Department of Plant Pathology, 201 Kottman Hall, 2021 Coffey Rd, Columbus, Ohio, United States, 43210; cohen.1107@osu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>The Ohio State University, 2647, Department of Plant Pathology, 201 Kottman Hall, 2021 Coffey Rd, Columbus, Ohio, United States</wicri:regionArea><wicri:noRegion>United States</wicri:noRegion></affiliation></author><author><name sortKey="Jacobs, Jonathan" sort="Jacobs, Jonathan" uniqKey="Jacobs J" first="Jonathan" last="Jacobs">Jonathan Jacobs</name><affiliation wicri:level="1"><nlm:affiliation>Ohio State University, 2647, Plant Pathology, 2021 Coffey Road, Columbus, Ohio, United States, 43210-1132; jacobs.1080@osu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Ohio State University, 2647, Plant Pathology, 2021 Coffey Road, Columbus, Ohio, United States</wicri:regionArea><wicri:noRegion>United States</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32997607</idno><idno type="pmid">32997607</idno><idno type="doi">10.1094/PHYTO-07-20-0273-SC</idno><idno type="wicri:Area/Main/Corpus">000086</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000086</idno><idno type="wicri:Area/Main/Curation">000086</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000086</idno><idno type="wicri:Area/Main/Exploration">000086</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The complete genome sequence of Xanthomonas theicola, the causal agent of canker on tea plants, reveals novel secretion systems in clade-1 xanthomonads.</title><author><name sortKey="Koebnik, Ralf" sort="Koebnik, Ralf" uniqKey="Koebnik R" first="Ralf" last="Koebnik">Ralf Koebnik</name><affiliation wicri:level="1"><nlm:affiliation>UMR IPME, 911, Avenue Agropolis, Montpellier, France, 34394; ralf.koebnik@ird.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>UMR IPME, 911, Avenue Agropolis, Montpellier, France</wicri:regionArea><wicri:noRegion>France</wicri:noRegion><wicri:noRegion>France</wicri:noRegion></affiliation></author><author><name sortKey="Burokiene, Daiva" sort="Burokiene, Daiva" uniqKey="Burokiene D" first="Daiva" last="Burokiene">Daiva Burokiene</name><affiliation wicri:level="1"><nlm:affiliation>Nature Research Centre, 282393, Institute of Botany, Laboratory of Plant Pathology, Vilnius, Lithuania; daiva.burokiene@gamtc.lt.</nlm:affiliation><country wicri:rule="url">Lituanie</country><wicri:regionArea>Nature Research Centre, 282393, Institute of Botany, Laboratory of Plant Pathology, Vilnius</wicri:regionArea><wicri:noRegion>Vilnius</wicri:noRegion></affiliation></author><author><name sortKey="Bragard, Claude" sort="Bragard, Claude" uniqKey="Bragard C" first="Claude" last="Bragard">Claude Bragard</name><affiliation wicri:level="4"><nlm:affiliation>Université catholique de Louvain, 83415, Earth and Life Institute, Louvain-la-Neuve, Walloon Brabant, Belgium; claude.bragard@uclouvain.be.</nlm:affiliation><country wicri:rule="url">Belgique</country><wicri:regionArea>Université catholique de Louvain, 83415, Earth and Life Institute, Louvain-la-Neuve, Walloon Brabant</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Chang, Christine" sort="Chang, Christine" uniqKey="Chang C" first="Christine" last="Chang">Christine Chang</name><affiliation wicri:level="1"><nlm:affiliation>Pacific Biosciences Inc, 105480, Menlo Park, California, United States; cchang@pacificbiosciences.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Pacific Biosciences Inc, 105480, Menlo Park, California</wicri:regionArea><wicri:noRegion>California</wicri:noRegion></affiliation></author><author><name sortKey="Fischer Le Saux, Marion" sort="Fischer Le Saux, Marion" uniqKey="Fischer Le Saux M" first="Marion" last="Fischer-Le Saux">Marion Fischer-Le Saux</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Recherche en Horticulture et Semences, 98754, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, Angers, Pays de la Loire, France; marion.le-saux@inrae.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Institut de Recherche en Horticulture et Semences, 98754, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, Angers, Pays de la Loire</wicri:regionArea><wicri:noRegion>Pays de la Loire</wicri:noRegion><orgName type="university">Université d'Angers</orgName><placeName><settlement type="city">Angers</settlement><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Kolliker, Roland" sort="Kolliker, Roland" uniqKey="Kolliker R" first="Roland" last="Kölliker">Roland Kölliker</name><affiliation wicri:level="1"><nlm:affiliation>ETH Zürich, 27219, 6 Institute of Agricultural Sciences, Molecular Plant Breeding, Zurich, ZH, Switzerland; roland.koelliker@usys.ethz.ch.</nlm:affiliation><country wicri:rule="url">Suisse</country><wicri:regionArea>ETH Zürich, 27219, 6 Institute of Agricultural Sciences, Molecular Plant Breeding, Zurich, ZH</wicri:regionArea><wicri:noRegion>ZH</wicri:noRegion></affiliation></author><author><name sortKey="Lang, Jillian" sort="Lang, Jillian" uniqKey="Lang J" first="Jillian" last="Lang">Jillian Lang</name><affiliation wicri:level="2"><nlm:affiliation>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado, United States; jillian.lang@colostate.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Leach, Jan" sort="Leach, Jan" uniqKey="Leach J" first="Jan" last="Leach">Jan Leach</name><affiliation wicri:level="2"><nlm:affiliation>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado, United States; Jan.Leach@colostate.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Luna, Emily" sort="Luna, Emily" uniqKey="Luna E" first="Emily" last="Luna">Emily Luna</name><affiliation wicri:level="2"><nlm:affiliation>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado, United States; emily.peachey@colostate.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Portier, Perrine" sort="Portier, Perrine" uniqKey="Portier P" first="Perrine" last="Portier">Perrine Portier</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Recherche en Horticulture et Semences, 98754, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, Angers, Pays de la Loire, France; perrine.portier@angers.inra.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Institut de Recherche en Horticulture et Semences, 98754, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, Angers, Pays de la Loire</wicri:regionArea><wicri:noRegion>Pays de la Loire</wicri:noRegion><orgName type="university">Université d'Angers</orgName><placeName><settlement type="city">Angers</settlement><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Sagia, Angeliki" sort="Sagia, Angeliki" uniqKey="Sagia A" first="Angeliki" last="Sagia">Angeliki Sagia</name><affiliation wicri:level="1"><nlm:affiliation>IRD Centre de Montpellier, 98751, UMR IPME, Montpellier, Occitanie, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IRD Centre de Montpellier, 98751, UMR IPME, Montpellier, Occitanie</wicri:regionArea><wicri:noRegion>Occitanie</wicri:noRegion><wicri:noRegion>Occitanie</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>University of Crete, 37777, Department of Biology, Heraklion, Crete, Greece; sagia.angeliki@gmail.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>University of Crete, 37777, Department of Biology, Heraklion, Crete</wicri:regionArea><wicri:noRegion>Crete</wicri:noRegion></affiliation></author><author><name sortKey="Ziegle, Janet" sort="Ziegle, Janet" uniqKey="Ziegle J" first="Janet" last="Ziegle">Janet Ziegle</name><affiliation wicri:level="1"><nlm:affiliation>Pacific Biosciences Inc, 105480, Menlo Park, California, United States; jziegle@pacificbiosciences.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Pacific Biosciences Inc, 105480, Menlo Park, California</wicri:regionArea><wicri:noRegion>California</wicri:noRegion></affiliation></author><author><name sortKey="Cohen, Stephen Philip" sort="Cohen, Stephen Philip" uniqKey="Cohen S" first="Stephen Philip" last="Cohen">Stephen Philip Cohen</name><affiliation wicri:level="1"><nlm:affiliation>The Ohio State University, 2647, Department of Plant Pathology, 201 Kottman Hall, 2021 Coffey Rd, Columbus, Ohio, United States, 43210; cohen.1107@osu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>The Ohio State University, 2647, Department of Plant Pathology, 201 Kottman Hall, 2021 Coffey Rd, Columbus, Ohio, United States</wicri:regionArea><wicri:noRegion>United States</wicri:noRegion></affiliation></author><author><name sortKey="Jacobs, Jonathan" sort="Jacobs, Jonathan" uniqKey="Jacobs J" first="Jonathan" last="Jacobs">Jonathan Jacobs</name><affiliation wicri:level="1"><nlm:affiliation>Ohio State University, 2647, Plant Pathology, 2021 Coffey Road, Columbus, Ohio, United States, 43210-1132; jacobs.1080@osu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Ohio State University, 2647, Plant Pathology, 2021 Coffey Road, Columbus, Ohio, United States</wicri:regionArea><wicri:noRegion>United States</wicri:noRegion></affiliation></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Xanthomonas theicola is the causal agent of bacterial canker on tea plants. There is no complete genome sequence available for X. theicola, a close relative of the species X. translucens and X. hyacinthi, thus limiting basic research for this group of pathogens. Here we release a high-quality complete genome sequence for the X. theicola type strain, CFBP 4691T. Single-molecule real-time sequencing with a mean coverage of 264X revealed two contigs of 4,744,641 bp (chromosome) and 40,955 bp (plasmid) in size. Genome mining revealed the presence of non-ribosomal peptide synthases, two CRISPR systems, the Xps type 2 secretion system, and the Hrp type 3 secretion system. Surprisingly, this strain encodes an additional type 2 secretion system and a novel type 3 secretion system with enigmatic function, hitherto undescribed for xanthomonads. Four type 3 effector genes were found on complete or partial transposons, suggesting a role of transposons in effector gene evolution and spread. This genome sequence fills an important gap to better understand the biology and evolution of the early-branching xanthomonads, also known as clade-1 xanthomonads.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32997607</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Sep</Month><Day>30</Day></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>The complete genome sequence of Xanthomonas theicola, the causal agent of canker on tea plants, reveals novel secretion systems in clade-1 xanthomonads.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-07-20-0273-SC</ELocationID><Abstract><AbstractText>Xanthomonas theicola is the causal agent of bacterial canker on tea plants. There is no complete genome sequence available for X. theicola, a close relative of the species X. translucens and X. hyacinthi, thus limiting basic research for this group of pathogens. Here we release a high-quality complete genome sequence for the X. theicola type strain, CFBP 4691T. Single-molecule real-time sequencing with a mean coverage of 264X revealed two contigs of 4,744,641 bp (chromosome) and 40,955 bp (plasmid) in size. Genome mining revealed the presence of non-ribosomal peptide synthases, two CRISPR systems, the Xps type 2 secretion system, and the Hrp type 3 secretion system. Surprisingly, this strain encodes an additional type 2 secretion system and a novel type 3 secretion system with enigmatic function, hitherto undescribed for xanthomonads. Four type 3 effector genes were found on complete or partial transposons, suggesting a role of transposons in effector gene evolution and spread. This genome sequence fills an important gap to better understand the biology and evolution of the early-branching xanthomonads, also known as clade-1 xanthomonads.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Koebnik</LastName><ForeName>Ralf</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>UMR IPME, 911, Avenue Agropolis, Montpellier, France, 34394; ralf.koebnik@ird.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burokiene</LastName><ForeName>Daiva</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Nature Research Centre, 282393, Institute of Botany, Laboratory of Plant Pathology, Vilnius, Lithuania; daiva.burokiene@gamtc.lt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bragard</LastName><ForeName>Claude</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Université catholique de Louvain, 83415, Earth and Life Institute, Louvain-la-Neuve, Walloon Brabant, Belgium; claude.bragard@uclouvain.be.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Christine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Pacific Biosciences Inc, 105480, Menlo Park, California, United States; cchang@pacificbiosciences.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fischer-Le Saux</LastName><ForeName>Marion</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Horticulture et Semences, 98754, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, Angers, Pays de la Loire, France; marion.le-saux@inrae.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kölliker</LastName><ForeName>Roland</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>ETH Zürich, 27219, 6 Institute of Agricultural Sciences, Molecular Plant Breeding, Zurich, ZH, Switzerland; roland.koelliker@usys.ethz.ch.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lang</LastName><ForeName>Jillian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado, United States; jillian.lang@colostate.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leach</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado, United States; Jan.Leach@colostate.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luna</LastName><ForeName>Emily</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Colorado State University, 3447, Department of Agricultural Biology, Fort Collins, Colorado, United States; emily.peachey@colostate.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Portier</LastName><ForeName>Perrine</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Institut de Recherche en Horticulture et Semences, 98754, Université d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, Angers, Pays de la Loire, France; perrine.portier@angers.inra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sagia</LastName><ForeName>Angeliki</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>IRD Centre de Montpellier, 98751, UMR IPME, Montpellier, Occitanie, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Crete, 37777, Department of Biology, Heraklion, Crete, Greece; sagia.angeliki@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ziegle</LastName><ForeName>Janet</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Pacific Biosciences Inc, 105480, Menlo Park, California, United States; jziegle@pacificbiosciences.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cohen</LastName><ForeName>Stephen Philip</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>The Ohio State University, 2647, Department of Plant Pathology, 201 Kottman Hall, 2021 Coffey Rd, Columbus, Ohio, United States, 43210; cohen.1107@osu.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jacobs</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Ohio State University, 2647, Plant Pathology, 2021 Coffey Road, Columbus, Ohio, United States, 43210-1132; jacobs.1080@osu.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacterial Pathogens</Keyword><Keyword MajorTopicYN="N">Evolution</Keyword><Keyword MajorTopicYN="N">Genomics</Keyword><Keyword MajorTopicYN="N">Host Parasite Interactions</Keyword><Keyword MajorTopicYN="N">Microbe-genome Sequencing</Keyword><Keyword MajorTopicYN="N">Pathogen Effectors</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>30</Day><Hour>17</Hour><Minute>11</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32997607</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-07-20-0273-SC</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li><li>France</li><li>Lituanie</li><li>Oman</li><li>Suisse</li><li>États-Unis</li></country><region><li>Colorado</li><li>Pays de la Loire</li><li>Province du Brabant wallon</li><li>Région wallonne</li></region><settlement><li>Angers</li><li>Louvain-la-Neuve</li></settlement><orgName><li>Université catholique de Louvain</li><li>Université d'Angers</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Koebnik, Ralf" sort="Koebnik, Ralf" uniqKey="Koebnik R" first="Ralf" last="Koebnik">Ralf Koebnik</name></noRegion><name sortKey="Fischer Le Saux, Marion" sort="Fischer Le Saux, Marion" uniqKey="Fischer Le Saux M" first="Marion" last="Fischer-Le Saux">Marion Fischer-Le Saux</name><name sortKey="Portier, Perrine" sort="Portier, Perrine" uniqKey="Portier P" first="Perrine" last="Portier">Perrine Portier</name><name sortKey="Sagia, Angeliki" sort="Sagia, Angeliki" uniqKey="Sagia A" first="Angeliki" last="Sagia">Angeliki Sagia</name></country><country name="Lituanie"><noRegion><name sortKey="Burokiene, Daiva" sort="Burokiene, Daiva" uniqKey="Burokiene D" first="Daiva" last="Burokiene">Daiva Burokiene</name></noRegion></country><country name="Belgique"><region name="Région wallonne"><name sortKey="Bragard, Claude" sort="Bragard, Claude" uniqKey="Bragard C" first="Claude" last="Bragard">Claude Bragard</name></region></country><country name="Oman"><noRegion><name sortKey="Chang, Christine" sort="Chang, Christine" uniqKey="Chang C" first="Christine" last="Chang">Christine Chang</name></noRegion><name sortKey="Sagia, Angeliki" sort="Sagia, Angeliki" uniqKey="Sagia A" first="Angeliki" last="Sagia">Angeliki Sagia</name><name sortKey="Ziegle, Janet" sort="Ziegle, Janet" uniqKey="Ziegle J" first="Janet" last="Ziegle">Janet Ziegle</name></country><country name="Suisse"><noRegion><name sortKey="Kolliker, Roland" sort="Kolliker, Roland" uniqKey="Kolliker R" first="Roland" last="Kölliker">Roland Kölliker</name></noRegion></country><country name="États-Unis"><region name="Colorado"><name sortKey="Lang, Jillian" sort="Lang, Jillian" uniqKey="Lang J" first="Jillian" last="Lang">Jillian Lang</name></region><name sortKey="Cohen, Stephen Philip" sort="Cohen, Stephen Philip" uniqKey="Cohen S" first="Stephen Philip" last="Cohen">Stephen Philip Cohen</name><name sortKey="Jacobs, Jonathan" sort="Jacobs, Jonathan" uniqKey="Jacobs J" first="Jonathan" last="Jacobs">Jonathan Jacobs</name><name sortKey="Leach, Jan" sort="Leach, Jan" uniqKey="Leach J" first="Jan" last="Leach">Jan Leach</name><name sortKey="Luna, Emily" sort="Luna, Emily" uniqKey="Luna E" first="Emily" 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